首款具备我国完全自主知识产权的高密度固相小麦育种芯片 (涵盖SNP标记、芯片、试剂及扫描仪)
华麦芯普通小麦基因分型65K芯片
合作单位
北京市农林科学院杂交小麦研究所、中国农业大学、西北农林科技大学等
华麦芯65K芯片严格遵照《小麦品种真实性鉴定SNP标记法》(NY/T 4021-2021),囊括真实性鉴定和已知品种高密度指纹数据库构建的所有位点,并整合转基因检测所必需的特定标记物,以及部分与小麦品质、抗性、产量等相关的功能基因或紧密连锁的标记。以中国春2.1版本为参考,目前包含54993个精选的分子标记(可根据未来研究重要结果扩充至65K),覆盖196个性状相关的标记。SNP位点在A、B、D染色体上分布较均衡,多态性位点占比高达99.127%,具备良好的区分能力和稳定性;单个SNP位点采用15-30个微球重复检测,平均检出率高达99.0%,复现性高达99.5%。
联系我们
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应用场景
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小麦品种真实性/特异性/一致性辅助检测
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实质派生品种诊断
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转基因检测
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遗传连锁图谱构建与QTL定位
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全基因组选择育种
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标记辅助选择育种
产品亮点及优势
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全自主
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自主研发并已获得专利授权
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主要供应商全部来自本土企业
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本土技术全流程支持
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可扩充
可根据用户需求和最新研究成果随时增加SNP位点。
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多效用
集背景筛选、品种鉴定、转基因检测等多用途于一体,可实现一站式全面筛查。
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低成本
规模化检测时展现出显著的成本效益。随着样本数量增加,单个样本的检测成本呈下降趋势。
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高效率
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从检测到数据分析,整个过程仅需72小时,且准确率保持在99%以上。
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仅需24个样本即可完成检测,无需凑样。
数据表现
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华麦芯65KSNP位点染色体分布图 本产品SNP位点丰富,完整覆盖普通小麦的21条染色体
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华麦芯65KSNP位点最小等 位基因频率(MAF)分布情况
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华麦芯65K检测187份审定品种 小麦标准样品构建的SNP指纹聚类图
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华麦芯65K检测测试样本的检出率 测试样本平均检出率为99.13%
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华麦芯65K检测测试样本的复现性 测试样本平均复现性为99.34%
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华麦芯65K单个SNP位点在 品种鉴定应用中的聚类分型图
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